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E.PereiraP.et al (2020)

Mechanism(s) regulating inhibition of thymidylate synthase and growth by - L -glutaminyl-4-hydroxy-3-iodobenzene, a novel melanin precursor, in melanogenic melanoma cells

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GST pulldown assay The prey proteins (PIF4, PIF4 mTAD , PIF4 mB , and PIF4 mBH ) were synthesized in vitro using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs Inc., Ipswich, MA) according to the manufacturers instructions