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StatPearls 35

In the Krebs cycle, NAD+ functions as a coenzyme for the rate-limiting enzymes alpha ketoglutarate dehydrogenase, isocitrate dehydrogenase, and malate dehydrogenase
After three washes with phosphate buffer, the samples were post-fixed with 1% osmium tetroxide for 2 h and dehydrated through a graded ethanol series at 4 C

doi: 10.1016/0003-2697(79)90738-3 44 PacherP.BeckmanJ

S.YariswamyM.KemparajuK.et al (2013)
