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It was observed that another prevalent motivation for choosing the GF diet was to lose weight, particularly among populations of young people and kids

casei by PCR using a pair of primer SOD_LC2W-F71/SOD_LC2W-R97 and inserted into pKLH71 and pKLH81 (digestion by Bam HI and Bgl II) to obtain pKLH295 and pKLH303, respectively, by the ClonExpress MultiS one-step cloning kit (Vazyme, Nanjing, China)

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