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2124 In the human adipocyte (Chub-S7) cell line, but also in primary cultures of human subcutaneous and omental adipocytes, cortisol and cortisone treatment decreased lipogenesis, demonstrated by decreased 14 C-acetate incorporation into lipid, in the context of reductions in acetyl coenzyme A carboxylase (ACC) mRNA expression and increases in the inactivating phosphorylation of the ACC protein

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Following treatment, the cells were harvested and subjected to centrifugation at 4000 rpm for 10 min using a benchtop swinging-bucket centrifuge