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The proliferation of progenitor cells including ASCs, stability of DNA, and morphological integrity are fundamental factors that impact ASCs stemness, pro-regenerative ability, and finally clinical usefulness [51,52,53]

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If freezing is necessary, aliquot the solution into single-use portions to avoid repeated freezing and thawing

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For localised injuries, injecting as close to the affected tissue as practical can improve outcomes significantly compared to a distant injection site
