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doi:10.1016/j.amjcard.2015.08.046 Selim BJ, Ramar K

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After blocking with 5% non-fat dry milk in TBST for 1 h at room temperature, the membranes were incubated overnight at 4 C with primary antibodies against ER (1:1000), IGF-1 (1:500), IGF-1R (1:1000), NKB (1:200), NK3R (1:100), and GnRH (1:1000), all purchased from Abcam (USA)
