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10.1016/j.cell.2005.05.007 193

Protein extraction and western blot analysis Cultured cells were lysed in radioimmunoprecipitation assay lysis buffer (Beyotime, Shanghai, China) supplemented with phenylmethylsulphonyl fluoride and protease inhibitor cocktail (Sigma-Aldrich, USA) on ice for 10 min and centrifuged at 12,000 g for 10 min at 4 C

[DOI] [PubMed] [Google Scholar] Jedlitschky G., Keppler D
Die Ergebnisse knnen je nach individuellem Gesundheitszustand variieren

However, insufficient evidence exists to justify the discontinuation of GLP-1 RAs before the procedure, and the ideal cessation timing for these agents remains indeterminate
