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After incubation at 37 C with shaking (500 rpm) for 1 h., the cells were pelleted at 5,000 x g for 10 min at 4 C, washed once with ice-cold phosphate-buffered saline (PBS, pH 7.4), resuspended in 200 L of cold TE lysis buffer (30 mM TrisHCl, 1 mM EDTA, pH 8.0), and transferred to Lysing matrix B tubes (MP Biomedical)

Moreover, in the presence of FA, ADH5 cells showed the early apoptosis marker phosphatidylserine (measured by Annexin V), a blockage of the cell cycle at G2/M phase, and sub-G1 DNA accumulation (Supplementary Fig

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