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Identity, structure, and function of the mitochondrial permeability transition pore: controversies, consensus, recent advances, and future directions

Brain tissue was homogenised with 1:10 (w/v) PBS on ice, then centrifuged at 3,000 rpm for 15 min at 4 C in an Eppendorf centrifuge and the supernatant was collected

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In nature, ALA is found only in the R form (Figure 1) and is present in the body both in free form and conjugated to conserved lysine residues in an amide bond, thus making this isomer a key cofactor in different biological systems [7]