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Library Preparation and Sequencing (1) Amplicon Purification PCR products ( 550 bp) were size-verified by 2% agarose gel electrophoresis and purified (TIANgel Midi Purification Kit, Tiangen, DP209, China)

Finally, the specific activity of PAL in a given sample was calculated by the formula provided in the kit instructions

After methyl transfer, SAM becomes S-adenosylhomocysteine, or SAH

D.GadalS.MhatreM.WilliamsonK

No Therapeutic Use Exemption pathway exists since AOD-9604 has no approved therapeutic indication
