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All primary antibodies were incubated overnight in 1% milk/TBST at 4C, followed by three washes with TBST for 10 min

Concluding remarks Initial mechanistic hypotheses concerning the molecular bases of opioid tolerance focused on homologous desensitization, viewing internalization as a key protective step for maintaining analgesic efficacy

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The model integrates selected remote elements including home-based laboratory sample collection, ePRO administration, telemedicine safety reviews, and electronic consent within a protocol that retains conventional investigational site visits for high-acuity or safety-critical procedures

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