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Use the cream every night for consistent results

After 45 min incubation, the resultant biofilms were washed with pre-warmed RPMI 1640 (x3), before adding a further 200 l of fresh medium and incubating for a further 24 h (20 rpm, 37C)

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10.18632/AGING.100168 [DOI] [PMC free article] [PubMed] [Google Scholar] Goldenbogen B, Giese W, Hemmen M, Uhlendorf J, Herrmann A, Klipp E (2016) Dynamics of cell wall elasticity pattern shapes the cell during yeast mating morphogenesis

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