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4.8 Assessment of oxidative stress parameters 4.8.1 Detection of reactive oxygen species (ROS) levels To evaluate intracellular ROS content, RBCs, left untreated or exposed to AAPH-containing solution (for 1 h, at 37C) with or without pre- or post-treatment with anthocyanin-enriched fraction (0.01 g/mL, for 1 h, at 37C) were incubated in Hanks balanced salt solution, pH 7.4, containing dihydrorhodamine 123 (DHR 123

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23 In rare cases, HG patients can develop severe consequences such as acute kidney injury secondary to hypovolemia and arrhythmiarelated cardiac arrest secondary to severe hypokalemia