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K., Dunphy, G., Heras-Murillo, I., Mastrangelo, A
Potentially, this may be associated with an increase in the steady-state level of HSPs and chronic activation of HIF-1 which activates the transcription of genes responsible for strengthening the intestinal barrier function
The lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C
doi: 10.1016/j.celrep.2021.108708 102 McklLMoernerWE
