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The bottom line Reconstitution seems complicated until you do it once

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DNA libraries were purified with AMPure XP beads (Beckman Coulter, Indianapolis, IN), indexed with Nextera XT DNA Library Preparation Kit (Illumina) and sequenced on the NGS MiSeq (Illumina) with MiSeq Reagent kit v2 (300 cycles) (Illumina)

Example: 20 mg peptide + 2 mL bacteriostatic water Concentration = 10 mg/mL If this entire 2 mL solution is transferred into a 3 mL cartridge, the concentration remains 10 mg/mL, not diluted

Initial loading protocols often use daily or every-other-day dosing for the first one to two weeks, followed by weekly, then monthly maintenance