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The optical properties of the carbon quantum dots are related to their particle size, modification, and heteroatom doping allowing them to be used in more applications [39]
qRT-PCR was carried out using SYBR Green Master Mix (cat

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doi: 10.1097/MIB.0000000000000389
However, since NEM is also a potent inhibitor of GR (which is required for the GSH-recycling assay), the procedure also calls for extraction of excess alkylating agent before proceeding with the GSSG titration
