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For detection of vinculin as loading control, membranes were blocked with Intercept Blocking Buffer (LI-COR Biosciences, #927-70001) for 1 h at room temperature, incubated with the primary antibody anti-vinculin (Sigma-Aldrich, V9131, RRID: AB_477629, 1:5000) overnight at 4 C in blocking buffer, then washed with PBS-T (PBS with 0.1% Tween-20) five times and incubated with IRDye 800CW-conjugated secondary antibody (LI-COR Biosciences, #926-32210, RRID: AB_621842, 1:5000) in blocking buffer for 1 h at room temperature with protection from light