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The USDA Agricultural Research Service used the 148 pmol/L threshold in their national prevalence studies, and the NIH Office of Dietary Supplements acknowledges that lab reference ranges can vary between institutions

PMID: 29155147): 5-Amino-1MQ showed NNMT selectivity with IC50 of 1.2 microM Reduced body weight and white adipose mass in diet-induced obese mice over 11 days No effect on food intake, suggesting metabolic rather than appetitive mechanism Nicotinamide N-methyltransferase inhibition mimics and boosts exercise-mediated improvements in muscle function in aged mice , published in Scientific Reports (Dimet-Wiley AL et al., 2024

However, trace amounts may be detectable longer depending on the sensitivity of testing methods