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[DOI] [PMC free article] [PubMed] [Google Scholar] 5.Gallogly MM, Starke DW, Mieyal JJ

Molecular docking with jr PPO1 and the five mutants Molecular docking was performed using AutoDock Vina 66 to identify binding poses of monophenolic (tyramine and L -tyrosine) and diphenolic substrates (dopamine and L -DOPA) within the active site of jr PPO1-wt and the five mutants in order to structurally analyse substrate binding and to identify differences between the wildtype and the mutants

After two washes with cold PBS, the cells were suspended in 195 L of binding buffer

Following brief agitation, each reconstituted supernatant was centrifuged again for 5 min at 4 C and 18,000 g prior to metabolite profiling

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