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After the last wash, cells were resuspended in DAPI mounting medium and seeded on Cell-Tak (Fisher Scientific 10317081) coated glass slides
The right hemisphere was then embedded in a mould of optimal cutting temperature compound (OCT, Scigen) and fast frozen in isopentane in a bath of liquid nitrogen until 2 min after the OCT had turned opaque
Abstract Acetaminophen (APAP) is a worldwide commonly used painkiller drug
