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the detection of the optical purity of L-3-cyano -2 - hydroxypropyl trimethyl ammoniumin and the content of its dextroisomer in step (3) of the present invention includes the following steps: L-carnitine which is started from S-epichlorohydrin (as shown in reaction formula I) provides a series of crucial quality control technique in different preparation steps, which can raise the optical purity of L-carnitine, reduce the content of harmful D-carnitine, low the cost effectively