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Conversely, estrogen exerts pro-inflammatory effects in specific autoimmune diseases (AIDs), including rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), highlighting estrogen as a risk factor for the increased incidence of various AIDs in women than in men (161, 163)

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A Spectra Multicolor Broad Range Protein Ladder (ThermoFisher) was used as standard, and the gels were stained with Comassie Brilliant Blue (BioRad)
