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Briefly, 2.5M perchloric acid was added to each half brain and the brains were roughly chopped using pointed surgical scissors

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The close associations of relatively abundant Stearic acid, Amiloride, and 3,4-Dimethoxyphenylethylamine with the identified OTUs, including Escherichia marmotae , Enterobacter hormaechei , Shigella boydii , Citrobacter koseri , and Subdoligranulum var iabile notably separated h-CKD groups from healthy controls

Studies in animal models suggest that ashwagandha has GABAergic properties, which would also contribute to its benefits