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Spectrum-centric workflows, such as DIA-Umpire 5 and Group-DIA 6 , generate pseudo-MS/MS spectra for each precursor from DIA data for routine data-dependent acquisition (DDA) database search by assembling precursor-fragment groups based on the elution profiles of precursor and fragment ions

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The concentration of Glutathione S Transferase mu can then be calculated by reading the O.D