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After 12 h of culture, cells were washed three times with 1 ml of ice-cold PBS and then the polar metabolites were extracted in 1.5 ml of 80% methanol (pre-chilled to 80 C)

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TOMM20 (ab56783) antibodies were purchased from Abcam (USA)

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However, improved CPT1A activity or NAD + -mediated SIRT activation may contribute to reducing oxidative stress and fatty acid accumulation by -oxidation stimulation

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Inflammatory and hormonal markers are assessed to understand how systemic inflammation, stress response, and hormonal balance may be influencing tissue repair, skin aging, or recovery processes

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