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These interference molecules will bind to streptavidin and prevent subsequent pairing of the donor and acceptor beads, which is indicated by a reduced AlphaScreen signal

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3.3 GDC-treated monolayers have a higher permeability to lactulose To evaluate whether decreased TEER values in GDC-treated monolayers corresponded to increased permeability, a lactulose permeability assay was performed on these monolayers (Figure 1C)