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Images were captured using confocal and inverted fluorescence microscopes

We used TGF-1 as a positive control because it is known to induce contraction of TM cells 29

This shields it from stomach acid and enhances absorption, delivering up to 4x more bioavailability than standard supplements

The third one (E3) is formed from E1 through 16-hydroxylation

Secondary antibodies against the species of primary antibodies were added and incubated for 1 h at room temperature
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