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A MiniTrap desalting column was used to change the buffer of the protein samples to 20 mM Na 2 HPO 4 , p H 7.5, 50 mM NaF, 5% ( w / v ) glycerol containing either 1 mM DTT or 0.5 mM GSH as reducing agents

Aoun, M., Karam, R., Sleilaty, G., Antoun, L., & Ammar, W

Moreover, the seal brain is capable of both producing and consuming lactate in vivo (Murphy et al., 1980), and glucose deprivation during hypoxia does not appear to negatively impact neuronal function in seal brain slices, suggesting that the seal brain may tolerate these insults (Czech-Damal et al., 2014

Charabati M, Wheeler MA, Weiner HL, Quintana FJ

This means that the variants that bind poorly to IGFPB have a short duration of action, faster onset of action and rapid withdrawal as compared to IGF-1 [4] In a study, researchers compared the hypoglycemic effects of IGF-1 and its variant IGF-1 Des in marmosets and pigs