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Modulation of Tyrosinase Expression Tyrosinase gene expression, together with Tyrp1 and Typr2, is induced by microphthalmia transcription factor (MITF), which is in turn regulated by signaling pathways, including cAMP/PKA/CREB, RAS/MEK/ERK, and -catenin pathways

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For immunofluorescence double staining with anti-FASN and anti-8-oxo-dG, paraffin sections were incubated with rabbit anti-FASN (dilution 1:50, Cell Signaling) and mouse anti-anti-8-oxo-dG (1:250, Trevigen) at 4 C o/n in a humidified chamber