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Description
A Waters 2777c UPLC system (Waters, USA) coupled with a Q Exactive HF high-resolution mass spectrometer (Thermo Fisher Scientific) was utilized for the isolation and identification of metabolites

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By inhibiting NNMT, 5-Amino-1MQ reduces 1-MNA formation and alleviates the methyl sink, increasing SAM availability for other methylation reactions, while freeing nicotinamide for recycling through NAMPT toward NAD
