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EC50 determination is reliable Scenario B: 85% pure IGF-1 LR3 stock (1 mg/mL) At a nominal concentration of 100 ng/mL in assay, actual IGF-1 LR3 = ~85 ng/mL Remaining ~15 ng/mL consists of potentially inhibitory, partially active, or bioactive contaminant species Dose-response curve is shifted

Peripheral neuropathy Antiretroviral-related peripheral neuropathy The use of certain antiretroviral agents (nucleoside analogs) has been associated with an increased risk of developing peripheral neuropathy in HIV-positive individuals (77, 78)

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Intraepidermal eosinophilic collections of basement membrane type IV collagen, denoted as caterpillar bodies, are frequently observed [62]