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Protein concentration was determined using a BCA protein assay kit (Thermo Fisher Scientific), and 20 g of protein per sample (the default amount if not specified otherwise) were subjected to SDS-PAGE, transferred onto PVDF membranes, and incubated with primary antibodies summarized in the key resources table, including GLUT1 (1:1000), MCT1 (1:1000), LDHB (1:1000), SOX2 (1:1000), GPR81 (1:1000), DNMT3B (1:1000), H3 (1:1000), H3K27m3 (1:1000), COX IV (1:1000), TOMM20 (1:1000), Na + -K + ATPase (1:1000), LAMP1 (1:1000), GM130 (1:1000), GRP78 (1:1000), -Tubulin (1:5000), and anti-CD147 (HAb18, 1:3000) produced in our laboratory 54,66

In AD, astrocytes assist in A removal via autophagy pathways [284] and activation of the urea cycle, notably through enhanced expression of ornithine decarboxylase 1, a key enzyme in polyamine biosynthesis [285]

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Radioprotective effect of Vitamins C and E

We quantitatively frame its hormetic behavior (a biphasic dose-response phenomenon characterized by low-dose stimulation and high-dose inhibition) and mechanistically explain its synergistic potential with conventional therapies