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The 16S rRNA gene V3-V4 region was amplified using a primer overhanging adapter (forward = 5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGC CTACGGGNGGCWGCAG-3 and reverse = 5-GTCTCGTGGGCTCGGAGATGTGTATAAGAG ACAGGACTACHVGGGTATCTAATCC-3)

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Symptomatic relief strategies include: Cold compresses : Applying a clean, cool (not ice-cold) compress to the injection site for 10-15 minutes can reduce inflammation, constrict blood vessels to decrease redness, and provide soothing relief from itching

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