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For immunoprecipitation samples, the database search was performed using the CHiMA 2.0 strategy 39

GR protein turnover is greatly stimulated in response to glucocorticoid binding through ubiquitination and subsequent proteasomal degradation ( 2.3 Glucocorticoid receptor physiology: molecular mechanisms of action 2.3.1 Genomic mechanisms of action GR is localized in the nucleus, cytosol, mitochondria, and cell membrane ( Figure 2 )

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Studies in immortalized cell lines indicate that internalization by SNC-80 is not followed by for receptor recycling [41] and in vivo experiments show that four hours after its systemic administration SNC-80-stimulated DORs remain trapped in the cytosol while analgesic tolerance is maximal [11]

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