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Analysis was conducted using an LC-MS QTRAP 6500+ system (SCIEX) equipped with a BEH C18 column (2.1 mm 10 cm, 1.7 m, Waters) and operated in ESI+ or ESI mode
Steady-state kinetics were measured using a pyrophosphate assay for the apo and AdoCbl-bound dark/light states of the Ab DPcob protein, as well as the truncated DGC domain only (Fig
doi: 10.3390/jcm8070938 91 KanagawaHMasuyamaRMoritaMSatoYNikiYKobayashiTet al
Shipton MJ, Thachil J
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