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The mitochondrial signal was binarized as well using a fixed thresholding approach and the nucleic acids dots contained in the detected mitochondrial staining were discarded

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Dacheux JL, Belleanne C, Jones R et al (2009) Mammalian epididymal proteome

And each mL of glutathione resin captures 8 mg of GST-fusion protein, so youll divide 100 mg by the binding capacity of 8 mg per mL of resin to calculate how much total resin you need