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Cell viability assay For cell viability assay, cells were planted in 96-well plates (34 10 3 per well) after indicated treatment, then incubated with 10% CCK-8 reagent at 37 C for 2 h
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29 This finding is in agreement with a recent in vivo study performed in humans, whereby overnight administration of hydrocortisone resulted in systemic insulin resistance, but enhanced insulin action in subcutaneous adipose tissue
