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It should also be noted that the younger and older division of the population in this study is arbitrary, merely based on participants mean age

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Deng S, Yang Y, He S, Chen Z, Xia X, Zhang T
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The 2D culture models of skeletal muscle cells have several advantages such as the simplicity and low-cost of maintaining cells in culture, the short time required to obtain the fusion of myoblasts into multinucleated myotubes (2 to 3 days) and the easy use of genetic manipulation [57, 97]
