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Complementary mechanisms GSH and TXN systems constitute the two thiol-based antioxidant networks that cooperate to restrain ferroptosis 115

Cell culture Swiss 3T3 fibroblasts, obtained from American Type Culture Collection (ATCC CCL 92), were maintained at 37 in Dulbecco's modified Eagle's medium supplemented with 25 mM HEPES (pH 7.4), 10% (v/v) fetal bovine serum, 100 units/mL penicillin, and 100 mg/mL streptomycin

In vitro and in vivo assessment of high-dose vitamin C against murine tumors

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Cell nuclei were stained with DAPI (dilution 1:5000, Invitrogen)