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[DOI] [PubMed] [Google Scholar] 75.McGill M.R., Lebofsky M., Norris H.R

HUVECs between passages 4 and 6 were cultured in a complete medium containing 53% M199 (Corning Incorporated), 37% human endothelial serum-free medium (Thermo Fisher Scientific), and 15 g/mL of endothelial cell growth supplement (Sigma-Aldrich)

In brief, the sheaths and stems of 2-week-old rice seedlings were cut into 0.5-mm strips and soaked in cell-wall digestion buffer (1.5% cellulase RS, 0.75% macerozyme R-10, 0.6 M mannitol, 10 mM MES, pH 5.7, 10 mM CaCl 2 , 0.1% BSA) for 6 h at 25 C in the dark with gentle shaking (4560 rpm)

Exposure to sublethal doses of fipronil and thiacloprid highly increases mortality of honeybees previously infected by Nosema ceranae