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Dispose of used needles according to facility safety protocols immediately

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Confocal and STED images were acquired using an Abberior microscope setup (Abberior Instruments GmbH) including Olympus IX83 microscope body and Imspector software (version 16.3.15521-w2209)

Population Doubling Level Calculation To calculate the PDL, a formula was used as follows: n = 3.32 (log UCY log I ) + X , where n is the final PDL at the end of a given subculture, UCY is the cell yield at that point, I is the cell number used as inoculum to begin that subculture, and X is the PDL of the inoculum used to initiate the subculture being quantitated ( SA--Galactosidase Staining Cells were seeded in 6-well microplates at 4 10 4 cells/well

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