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After a 30 minute incubation on ice, the blood was centrifuged at 1000 rpm for 15 min to separate the serum

Thiazolidinediones (TZDs) are PPAR ligands and act as insulin sensitizers that lower plasma glucose (2)

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Slow titration is used specifically to let the gut adapt and limit nausea
V.HabashiN.McCunnM.ShihD.McQuillanK.et al (2007)
