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Aliquots (3 L) of ethanolic cell pellet extracts were analysed by means of UPLC-TOF MS using the following solvent gradient for chromatography (0.4 mL/min, 45 C): starting with a mixture (10/90, v/v) of water and methanol, the methanol content was increased to 100% within 8 min and, then, kept constant for 1 min

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This is a known consideration in freeze-dried and frozen formulation research, where excipients such as mannitol are studied specifically to protect molecules through these phase changes, as covered in the Journal of Pharmaceutical Sciences review cited above