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Clone with a Q45R substitution showed the best activity and was named G2 (Supplementary Fig

Subsequently, 1 g of the obtained powder was extracted under optimized extraction conditions with 10 mL of 96% ethanol in an ultrasonic bath for 15 min at 45C

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One of their important functions is the metabolism of two neurotoxic agents: ammonia and glutamate, using the enzyme called glutamine synthetase (GS)

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