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From this, an aliquot (1 ml) was added to either 1 l unlabeled Luria-Bertani medium or 1 l 15N labelled M9 minimal medium (100 mM KH 2 PO 4 , 50 mM K 2 HPO 4 , 60 mM Na 2 HPO 4 , 14 mM K 2 SO 4 , 5 mM MgCl 2
