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Importantly, GLP-1 analogs appear to exert their anti-obesity effects through central pathways that do not involve MC4R, as well as via peripheral mechanisms involving the vagus nerve [48, 49]

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Furthermore, as hormones can also affect hair growth [60], serum T and E2 levels in the mice were measured using ELISA kits, and the results indicated no significant variations in the T/E2 ratios in mice treated with CaT-ME or CaT, implying that these systems did not alter hormonal homeostasis in mice (Figure S10 and 11)