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Qi Y, Liang Z, Wang Z et al (2015) Determination of Rab5 activity in the cell by effector pull-down assay
Evidence-based Complementary Alternative Medicine eCAM 306705
The protein mixture was concentrated 10-fold by using Amicon Ultra protein concentrators (MilliporeSigma, UFC8010, 10 kDa MWCO)
Active site residues U46, R48, Q81, and W136 are highlighted and shown as sticks
ELISA assays complement the HPLC analysis by measuring concentrations of melatonin and neurotrophic factors, utilizing kits from Sangon Biotechnology Company (Shanghai, China) following strict protocols to ensure reliability
