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They were then supplemented with DMEM medium containing 10% fetal bovine serum and 1% penicillin-streptomycin

Amino acid equilibration experiments and LC-MS To study changes in cellular amino acid equilibria, A549 and U87-MG cells were incubated in BME with 1 mM pyruvate and nonessential amino acids, including glutamine at 0.6 mM, or a modified HBSS solution (supplemented with 23 mM NaHCO 3 and with all proteinogenic amino acids at a baseline concentration of 100 M)

CrossRef A New Histone Deacetylase Inhibitor, MHY219, Inhibits the Migration of Human Prostate Cancer Cells via HDAC1 Umasankar De, Soma Kundu, Nabanita Patra, Mee Young Ahn, Ji Hae Ahn, Ji Yeon Son, Jung Hyun Yoon, Hyung Ryoung Moon, Byung Mu Lee, Hyung Sik Kim Biomolecules & Therapeutics.2015

Nature 2016;535:5664

This notion is supported by the observation that restriction of heme release using different approaches, such as binding of Hb to Hp or strengthening the globin-heme binding, inhibits the deleterious effects of these Hb species (Balla et al., 2002
