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Cell preparation for confocal microscopy DBCO-labeled cells prepared as described earlier were washed 3X with PBS and resuspended in the same volume of PBS as was drawn from the original cell suspension
Next, the sections were incubated with horseradish peroxidase-conjugated anti-rabbit antibodies (Alpha Diagnostic, San Antonio, TX, United States) for 20 min in a 37C incubator and then rinsed with PBS three times for 5 min each
The GST tag, due to its properties, facilitates a range of experimental techniques and applications
The key epigenetic regulatory mechanisms including DNA methylation, post-translational histone modifications, and the functions of non-coding RNAs, are critically involved in the initiation, maintenance, and progression of microvascular and macrovascular complications in DM [106, 107]
doi: 10.1016/j.scitotenv.2023.167202 59 ZhaiXLinYZhuLWangYZhangJLiuJet al
