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Absorbance of reaction mixture was recorded at 734 nm by Spectrophotometer (Jenway 3300, UK) and percent inhibition was calculated by the following formula: Ferric reducing antioxidant power assay (FRAP) FRAP reagent was made by adding acetate buffer (300 mM, pH 3.6), TPTZ (10 Mm) in HCl (40 Mm), and ferric chloride (0.054 g, 20 mM)
Forbes and Thorburn 2018)
tumefaciens and Prevotella metabolize isobutyric, butyric, and valeric acids (Wang et al., 2018)
Recently, SLC1A3, as a crucial effector of p53, has been shown to support cell survival and growth in the absence of glutamine 149
